新医学Issue(7)：435-439,5.DOI:10.3969/g.issn.0253-9802.2014.07.004
重型β地中海贫血儿童自然杀伤细胞活性及受体表达分析
Analysisi of the activity and receptor expression of peripheral NK cells in children with β-thalassemia major
鲍俊杰 1郑常龙 2何映谊 3丘媛媛 1廖思红 4李芳4
作者信息
- 1. 510180 广州，广州市妇女儿童医疗中心检验部
- 2. 510630 广州，中山大学附属第三医院急诊科
- 3. 510180 广州，广州市妇女儿童医疗中心 血液科
- 4. 510630 广州，中山大学附属第三医院 输血科
- 折叠
摘要
Abstract
Objective To analyze the activity and receptor expression of peripheral natural killer (NK)cells in children with β-thalassemia major and investigate underlying mechanism of the transfusion-in-duced immunodepression. Methods Peripheral blood samples were obtained from 20 children withβ-thalasse-mia major,20 β-thalassemia minor and 20 healthy volunteers. Expression levels of CD107a,activating recep-tors (NKP30,NKP46 and NKG2D)and inhibitory receptors (NKG2A and CD158a)within circulating NK cells were detected by multicolor flow cytometry. The level of IFN-γsecreted by NK cells was measured by in-tracellular cytokine staining. Results Compared with β-thalassemia minor and healthy counterparts (all P<0.05),the cytotoxicity (CD107a expression)of circulating NK cells was remarkably down-regulated whereas NKG2A expression,as an inhibitory receptor of circulating NK cells,was significantly up-regulated in children with β-thalassemia major (all P<0.05 ). However,no statistical significance was observed in the expression of NKP30,NKP46,NKG2D,CD158a and IFN-γ(all P>0.05)among three groups. Conclusion NKG2A, as an inhibitory receptor of NK cells,may play a key role in the transfusion-induced immunodepression of NK cells in children with β-thalassemia major.关键词
输血/β地中海贫血/自然杀伤细胞/自然杀伤细胞抑制性受体Key words
Transfusion/β-thalassemia/Natural killer cell/Natural killer cell inhibitory receptor引用本文复制引用
鲍俊杰,郑常龙,何映谊,丘媛媛,廖思红,李芳..重型β地中海贫血儿童自然杀伤细胞活性及受体表达分析[J].新医学,2014,(7):435-439,5.
