新医学Issue(7)：472-476,5.DOI:10.3969/g.issn.0253-9802.2014.07.013
类风湿关节炎患者外周血IL-25的表达及临床意义
Expression level and clinical significance of interleukin-25 in peripheral blood of rheumatoid arthritis patients
唐宏勇 1李忆农 2洪春梅1
作者信息
- 1. 350000 福州，福建医科大学福总临床医学院 南京军区福州总医院 风湿免疫科
- 2. 350000 福州，南京军区福州总医院风湿免疫科
- 折叠
摘要
Abstract
Objective To measure the expression levels of interleukin (IL)-25 and IL-17 in pe-ripheral blood of patients with rheumatoid arthritis (RA)and analyze the relationship between IL-25 ,and clini-cal index and the disease activity index 28 (DAS 28)scores in RA patients. The aim of this study is to identify the clinical significance of IL-25 in RA patients. Methods Peripheral blood samples were collected from 80 RA patients and 30 healthy volunteers. All subjects were above 16 years old. Fifty RA patients with DAS 28 score>3.2 were assigned into the RA active group and 30 with DAS 28 score≤3.2 were allocated into the RA inactive group. Hands joint X-ray total Sharp score and Health Assessment Questionnaire (HAQ)were recor-ded. The mRNA levels of IL-25/IL-25R and IL-17 in the peripheral blood mononuclear cells (PBMC)were determined by quantitative real-time PCR (QRT-PCR). The serum IL-25 and IL-17 proteins were detected by enzyme-linked immunosorbent assay (ELISA ). One-way ANOVA,Spearman’s or Pearson’s correlation and multiple linear regression were adopted to perform statistical analysis. Results The serum IL-25 and IL-17 proteins in the RA active group was significantly higher than those in the RA inactive and control groups (P<0.01),whereas no statistical significance was noted between the RA inactive and control groups (P>0.05).The mRNA levels of IL-25 and IL-17 in the RA active group were higher compared with those in the RA inac-tive and control groups (P<0.01),whereas no significant difference was found between the RA inactive and control groups (P>0.05 ). The potein level of serum IL-25 was positively correlated with the levels of CRP,ESR,RF,anti-CCP antibody,DAS 28,total Sharp score,HAQ (P<0.01 ),and the same outcome was found in the mRNA levels of IL-25 in PBMC (P<0.05 ). Both mRNA and protein levels of IL-25 were posi-tively correlated with corresponding IL-17 levels (b=0.748,P<0.01;b=0.843,P<0.01). Conclusion IL-25 in PBMC was probably involved with RA immune inflammation and it may serve as indicator of the activi-ty of RA.关键词
关节炎,类风湿/白细胞介素-25/相关性Key words
Arthritis/Rheumatoid/IL-25/Correlation引用本文复制引用
唐宏勇,李忆农,洪春梅..类风湿关节炎患者外周血IL-25的表达及临床意义[J].新医学,2014,(7):472-476,5.
