新医学Issue(9)：593-596,4.DOI:10.3969/g.issn.0253-9802.2014.09.007
N-乙酰半胱氨酸对大鼠心脏移植缺血再灌注损伤时基质金属蛋白酶的影响
Effects of N-acetylcysteine on modulating matrix metalloproteinase in myocardial ischemia reperfusion injury during heart transplantation in rats
摘要
Abstract
Objective To explore the protective effect of N-acetylcysteine (NAC)on modulating ma-trix metalloproteinase (MMP)in myocardial ischemia reperfusion injury (IRI)during heart transplantation in rat models.Methods Sixty healthy male Lewis rats were randomly divided into three groups.Control group:0.5 ml Saline was infused via inferior vena cava 30 min before donor harvesting;Donor pretreatment group:NAC (300 mg/kg BW)was infused via inferior vena cava 30 min before donor harvesting;Recipient pretreat-ment group:NAC (300 mg/kg BW)was infused via inferior vena cava 30min before recipient implantation.Grafts were kept in cold storage in conventional histidine tryptophan ketoglutarate (HTK)solution at 4℃ for 1 8 hrs prior to heterotopic transplantation.Heart transplantation was performed in each group.Tissue samples were taken 24 hrs after reperfusion for measurement of tissue collagen contents,MMP2 /9 protein expression,and MMP2 /TIMP2 mRNA expression.Tissue collagen contents were detected by sirus red.MMP2 /9 protein ex-pression was detected by immunohistochemistry and MMP2 /TIMP2 mRNA expression was measured by real-time PCR.Results Tissue collagen contents in donor/recipient pretreatment group significantly decreased compared with that in control group (4.49 ±0.62 /5.88 ±0.96 vs.1 0.43 ±0.93,P <0.01 ).MMP2 /9 pro-tein expression in donor/recipient pretreatment group was significantly lower compared with that in control group (1 .60 ±0.22 /2.1 3 ±0.23,1 .63 ±0.1 8 /2.78 ±0.1 6 vs.3.00 ±0.1 5 /3.78 ±0.1 5,P <0.01 ),as illustra-ted in Table 3.Real-time PCR revealed that the level of MMP2 mRNA in recipient pretreatment group was sig-nificantly lower compared with that in control group (1 .45 ±0.72 vs.2.77 ±1 .62,P <0.05),whereas the expression of TIMP2 mRNA in donor pretreatment group was significantly higher than that in control group (2.59 ±2.1 2 vs.0.63 ±0.50,P <0.05).Conclusion NAC can protect graft cardiac function after heart transplantation,probably by directly suppressing reactive oxygen species-induced MMP2 /9 evelation and up-regulating TIMP2 activity.关键词
N-乙酰半胱氨酸/心脏移植/缺血再灌注损伤/基质金属蛋白酶/基质金属蛋白酶抑制剂Key words
N-acetylcysteine/Heart transplantation/Ischemia reperfusion injury/Matrix metallopro-teinase/Tissue inhibitor of metalloproteinase引用本文复制引用
伊雪,李占清,杨述亮,邬鹏宇,王帅,崔翔宇,关晓海,杨学慧,郑素琴..N-乙酰半胱氨酸对大鼠心脏移植缺血再灌注损伤时基质金属蛋白酶的影响[J].新医学,2014,(9):593-596,4.基金项目
河北省留学人员科技活动项目择优资助项目 （）
