新医学Issue(7)：428-432,5.DOI:10.3969/g.issn.0253-9802.2015.07.003
miRNA-132通过靶向调控 Bmi-1表达影响鼻咽癌细胞对放射治疗敏感性
miRNA-132 affects the sensitivity of nasopharyngeal carcinoma cells to radiotherapy via targeted regu-lation of Bmi-1 expression
谭洁媚 1李明毅 1梁颖2
作者信息
- 1. 529031 江门，广东省江门市中心医院 中山大学附属江门医院肿瘤科
- 2. 510000 广州，中山大学肿瘤防治中心内科
- 折叠
摘要
Abstract
Objective To investigate the effect of miRNA-132 on the sensitivity of nasopharyngeal carcinoma (NPC)cells to radiotherapy.Methods The expression levels of miRNAs were compared between 5-8F parental cells and 5-8F /R radioresistant cells using a miRNA array;miRNA-132,which showed the most prominent changes between the two cell lines,was chosen for further study.The effect of miRNA-132 on apop-tosis was examined using flow cytometry.A dual-luciferase reporter assay and western blotting were employed for the identification and verification of the downstream targets of miRNA-132.Results miRNA-132 promoted apoptosis,enhanced radiosensitivity in 5-8F /R cells and inhibited the in vivo growth of 5-8F /R cells.In addi-tion,miRNA-132 suppressed Bmi-1 expression at both the mRNA and protein levels.Conclusion miRNA-132 enhanced the radiosensitivity of NPC cells via negative regulation of its downstream target Bmi-1.关键词
miRNA-132/Bmi-1/鼻咽癌/放射治疗增敏Key words
miRNA-132/Bmi-1/Nasopharyngeal carcinoma/Radiosensitization引用本文复制引用
谭洁媚,李明毅,梁颖..miRNA-132通过靶向调控 Bmi-1表达影响鼻咽癌细胞对放射治疗敏感性[J].新医学,2015,(7):428-432,5.
