烟草科技Issue(1)：59-65,7.DOI:10.16135/j.issn1002-0861.20150110
卷烟烟气丙烯酰胺暴露量的生物标志物分析及与滤嘴分析法的比较
Assessment of Acrylamide Exposure During Cigarette Smoking by Biomarker Analysis and Comparison with Filter Analysis Method
摘要
Abstract
In order to assess the exposure to acrylamide of a smoker during smoking accurately, an SPE-HPLC-MS/MS method was developed for determining the mercapturic acid (MA) metabolites, N-acetyl-S-(2-carbamoylethyl)-L-cysteine (AAMA) and N-acetyl-S-(2-carbamoyl-2-hychoxyethyl)-L-cysteine(GAMA), of acrylamide in human urine.The contents of AAMA and GAMA in the urine of smokers and nonsmokers were determined, and the correlation analysis between the determined data and the acrylamide exposure evaluated by filter analysis was conducted. The results showed that: 1)The method was accurate and sensitive with the intra-day and inter-day precisions less than 7% and 11%, respectively. The recoveries ranged from 94.9%to 100.0%, and the detection limits of AAMA and GAMA were 0.6 and 0.1 ng/mL, respectively. 2) The mean value of AAMA and GAMA in smokers��urine was three times the value in nonsmokers’. 3) The content of AAMA, the main metabolite of acrylamide, in urine had a certain correlation(R=0.567, P<0.03)with the acrylamide exposure determined by filter analysis.关键词
卷烟烟气/LC-MS/MS/丙烯酰胺/生物标志物/AAMA/GAMA/滤嘴分析法Key words
Cigarette smoke/LC-MS/MS/Acrylamide/Biomarker/AAMA/GAMA/Filter analysis分类
轻工纺织引用本文复制引用
王冰,赵阁,余晶晶,王昇,蔡君兰,于永杰,谢复炜..卷烟烟气丙烯酰胺暴露量的生物标志物分析及与滤嘴分析法的比较[J].烟草科技,2015,(1):59-65,7.基金项目
国家科技支撑计划项目“食品中热反应伴生危害物毒理学检测及风险苹果关键技术研究”（2012BAK01B03）。 （）
