烟草科技Issue(5)：23-27,5.DOI:10.16135/j.issn1002-0861.20150505
烟草NtMAPK基因克隆及激素诱导条件下的表达分析
Cloning of NtMAPK Gene in Tobacco and Analysis on its Expression Induced by Plant Hormone
摘要
Abstract
Mitogen-activated protein kinase (MAPK) is a key component in signal substance in plant’s defensive reaction and an important signal molecule in response to abiotic and biotic stresses. To reveal the function of its homolog genes in tobacco, a homolog sequence of MAPK was cloned from Nicotiana tabacum and named as NtMAPK. Amino acid sequence alignment analysis showed that NtMAPK gene belonged to MAPK family of cultivated tobacco. Phylogenetic analysis indicated that NtMAPK featured close phylogenetic relationship with rice OsMPK2/OsMPK6, it suggested that it might function similarly to OsMPK6 and related to plant’s resistance to pathogens. Under the induction of plant defensive reaction-related signals, semi-quantitative PCR analysis revealed that the expression of NtMAPK was up-regulated by ABA, MeJA and SA.关键词
烟草/促分裂素原活化蛋白激酶(MAPK)/克隆/表达Key words
Tobacco/MAPK/Clone/Expression分类
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熊海军,张兴坦,吴向伟,高军平,谢小东,王根洪,夏庆友..烟草NtMAPK基因克隆及激素诱导条件下的表达分析[J].烟草科技,2015,(5):23-27,5.基金项目
中国烟草总公司烟草基因组计划重大专项项目“烟草基因组编辑技术的建立与应用”［110201301011A （JY-11A）］；云南省烟草公司科技计划项目“烟草规模化基因功能鉴定及应用”（2013YN08)。 （）
