烟草科技Issue(6)：1-8,8.DOI:10.16135/j.issn1002-0861.20150601
烟草牻牛儿基牻牛儿基焦磷酸合成酶基因家族的全基因组鉴定
Whole Genome Identification and Analysis of Tobacco GGPP Synthase Gene Family
摘要
Abstract
In order to reveal the characteristics and functions of GGPPS (Geranylgeranyl pyrophosphate synthase)gene family in tobacco, the GGPPS family in Nicotiana tabacum(N. tabacum), Nicotiana sylvestris (N. sylvestris) and Nicotiana tomentosiformis(N. tomentosiformis) were searched, identified and analyzed in whole genome level through in silico methods. Nine members (seven large subunits and two small subunits) in N. tabacum, and five ones (four large subunits and one small subunit) in N. sylvestris and N. tomentosiformis were identified respectively. The open reading frames of GGPPS were 999-1 119 bp in <br> length, coded 333-372 amino acids with protein molecular weights of 36.3-40.7 kDa and pI in the range of 5.41-8.32. Small subunit genes consisted of two exons, while there was only one exon in large subunit genes. Both genes comprised DD(XX)1-2D and CXXXC conservative motifs, though certain differences existed. In the course of evolution, GGPPS family in tobacco might have undergone both tandem duplication and segmental duplication, and gene loss event probably occurred in N. tabacum. In addition, NtabGGPPS1-1 and NtabGGPPS1-2 evolved faster than the other ones and might be under purifying selection.关键词
普通烟草/林烟草/绒毛状烟草/牻牛儿基牻牛儿基焦磷酸合成酶(GGPPS)/基因家族/全基因组Key words
Nicotiana tabacum/Nicotiana sylvestris/Nicotiana tomentosiformis/Geranylgeranyl pyrophosphate synthase(GGPPS)/Gene family/Whole genome分类
轻工纺织引用本文复制引用
李泽锋,林福呈,李锋,魏攀,夏玉珍,王中,武明珠,王燃,罗朝鹏,金立锋,杨军..烟草牻牛儿基牻牛儿基焦磷酸合成酶基因家族的全基因组鉴定[J].烟草科技,2015,(6):1-8,8.基金项目
中国烟草总公司郑州烟草研究院科技项目“烟草萜类化合物合成关键基因Ntggpps功能分析和调控机制研究”（902012CZ340）。 （）
