烟草科技Issue(6)：40-44,5.DOI:10.16135/j.issn1002-0861.20150608
UPLC-Q Exactive轨道阱高分辨质谱法测定小鼠血液中的NNN及其7种代谢物
Determination of NNN and its Seven Metabolites in Mouse Blood with UPLC-Q Exactive Orbit Trap High-Resolution Mass Spectrometry
摘要
Abstract
In order to accurately determine the N-nitrosonornicotine (NNN) and its metabolites in mouse blood, a UPLC-Q Exactive orbit trap high-resolution mass spectrometry method was established by optimizing chromatographic and mass spectrometric conditions and using NNN-d4 and HPB-d4 as internal standards. Wherein, after protein precipitating with acetonitrile, the sample was separated directly by an HILIC column in UPLC spectrometer. NNN and its metabolites in mouse plasma were analyzed by mass spectrometry under full scan/data-dependent MS2(FS/ddMS2)mode. The results showed that: 1)The limits of detection and the recoveries of NNN and its metabolites ranged from 0.019 to 0.067 ng/mL and from 78.0% to 117.5%, respectively, with the intra-day precision of 0.47%-8.38%. 2)The developed method was suitable for the simultaneous analysis of NNN and its seven metabolites in mouse plasma.关键词
烟草特有亚硝胺/N-亚硝基降烟碱/小鼠/血液/代谢物/Q Exactive轨道阱高分辨质谱Key words
TSNA/N-Nitrosonornicotine(NNN)/Mouse/Blood/Metabolite/Q Exactive orbit trap high-resolution mass spectrometry分类
医药卫生引用本文复制引用
冯明飞,刘俊辉,卢斌斌,孙世豪,洪广峰,刘帅东,张建勋..UPLC-Q Exactive轨道阱高分辨质谱法测定小鼠血液中的NNN及其7种代谢物[J].烟草科技,2015,(6):40-44,5.基金项目
国家自然科学基金面上项目“改性介孔材料固相萃取/UPLC-MSn方法研究烟草特有亚硝胺在动物体内的代谢”（21175153）；中国烟草总公司郑州烟草研究院院长科技发展基金项目“MIPs-SPE-HILIC-MSn在线分离鉴定平台的搭建及其在TSNAs分析中的应用”（602011CA0310）。 （）
