岩矿测试Issue(6)：885-891,7.DOI:10.15898/j.cnki.11-2131/td.2014.06.020
加速溶剂萃取技术提取海洋沉积物中游离态脂肪酸的方法研究
Study on Accelerated Solvent Extraction of Free Fatty Acids in Marine Sediments
摘要
Abstract
Fatty acids are one of the most abundant lipid markers in marine sediments,whose primary occurrence forms are free fatty acids and conjugated fatty acid.Free fatty acid can be extracted directly by solvent,and conjugated fatty acid is extracted from leftover sediment residue after free fatty acid has been extracted,through adding acid or alkali,and then extracting after being heated.Accelerated Solven Extraction (ASE)is a very popular method in the study of fatty acids due to its low cost and low toxicity.In this study,coastal sediments were collected from the East China Sea.Conjugated fatty acid is not suitable for verifying the method due to its low concentration and large uncertainty;therefore,the determination of free fatty acid in marine sediments by Gas Chromatography-Mass Spectrometry with ASE for sample pretreatment is discussed in this paper.The main factors which affect the extraction yield of fatty acid by ASE,such as the proportion of extractants,the temperature,static extraction time and cycle times are discussed and optimized.The following conclusions were reached:methanol-methylene chloride (V/V=1 ∶3)is more suitable to be the extractant.The extraction efficiency of different types of fatty acids varies with increasing temperature,static extraction time and cycle times.Based on increasing carbons being extracted and the highest extraction efficiency,the optimum parameters for fatty acid extraction from marine sediments are:extraction temperature of 1 1 0℃,static time of 1 0 min and 3 cycles.The precisions of this method were 3.3%to 1 9.0%RSD for most free fatty acids,and the recovery rates were from 72.2% to 1 04.6%.This method is applicable to the quantitative analysis of alcohols and ketones in marine sediment,which is an improvement on conventional Soxhlet extraction with less reagent consumption,good precision and is a simple procedure.关键词
海洋沉积物/游离态脂肪酸/甲醇-二氯甲烷/加速溶剂萃取Key words
marine sediments/free fatty acids/methanol-methylene chloride/Accelerated Solvent Extraction分类
化学化工引用本文复制引用
李小琳,贺行良,李凤,张媛媛,陈立雷,张培玉..加速溶剂萃取技术提取海洋沉积物中游离态脂肪酸的方法研究[J].岩矿测试,2014,(6):885-891,7.基金项目
国土资源部海洋油气资源和环境地质重点实验室基金 （）
