临床医学工程Issue(7)：829-831,3.DOI:10.3969/j.issn.1674-4659.2014.07.0829
IFN-γ诱导THP-1源巨噬细胞活化的条件优化
Optimization of IFN-γ-Induced THP-1 Macrophage Activation
摘要
Abstract
Objective To find out the optimal cytokines and detection time when the activation state of macrophages derived from THP-1 induced by IFN-γ was detected. Methods THP-1 cells which differentiated into macrophages induced by phorbol ester (PMA), IFN-γwere added to induce the activation of macrophages for twice. ELISA was used to detect the concentration of TNF-α, IL-10, IL-12 and IL-23 in cultured supernatant in different stimulation concentration and stimulation time. Results The macrophages induced by IFN-γcould secrete a mass of TNF-αwhich could increase to over 1 000 ng/mL. At the same time, there were no obvious concentration changes of IL-10, IL-12 and IL-23. The minimum concentration of IFN-γto induce macrophages activation was 2.5 ng/mL for each time. The optimal first stimulation time was 8 to 16 hours while the optimal second stimulation time was 6 to 18 hours. Conclusions Macrophages can be induced by IFN-γ. Stimulation by IFN-γfor twice is more effective than once, therefore, it can be an ideal detection index for macrophage activation.关键词
巨噬细胞/IFN-γ/TNF-α/活化Key words
Macrophages/IFN-γ, TNF-α/Activation分类
医药卫生引用本文复制引用
江勇,钟球,陈涛,郭卉欣,赖赛麟,钱明,李海成,黎贞燕,余丽,江振友,周琳..IFN-γ诱导THP-1源巨噬细胞活化的条件优化[J].临床医学工程,2014,(7):829-831,3.基金项目
国家“十二五”传染病科技重大专项“纳米技术检测白介素-22等诊断结核潜伏感染的研究”（2014ZX10003002-003）；广东省“十二五”医学重点实验室-结核病防治与转化基金 （）
