疑难病杂志Issue(5)：509-512,4.DOI:10.3969/j.issn.1671-6450.2014.05.022
外周血内皮祖细胞移植对肺动脉高压兔模型肺动脉压力及其结构的影响
Influence of peripheral blood endothelial progenitor cells transplantation on pulmonary artery pressure and structure in rabbit model of pulmonary hypertension
摘要
Abstract
Objective To study therapeutic effect of peripheral blood endothelial progenitor cells ( EPCs) transplan-tation on pulmonary arterial hypertension in rabbit model induced by monocrotaline .Methods 50 healthy New Zealand rabbits were selected and randomly divided into 3 groups:(1)normal control group ( n =16), (2)pulmonary arterial hypertension group induced by monocrotaline (MCT model group, n =16),(3) EPCs treatment group ( n =18).Rabbits peripheral blood mononuclear cells were induced and differentiated into EPCs ,then injected into the tail vein of rabbit in EPCs treatment group , same volume of normal saline were injected in normal control group and MCT model group .After 3 weeks, pulmonary artery pressure, right ventricular hypertrophy index and pulmonary vascular remodeling were observed and compared .Results Com-pared with normal control group , pulmonary artery pressure of MCT model group increased (30.13 mm Hg ±3.12 mm Hg vs. 15.99 Hg mm ±2.23 mm Hg), right ventricular hypertrophy index increased (0.51 ±0.06 vs.0.26 ±0.03), pulmonary artery wall thickness increased (28.77μm ±4.35μm vs.8.44μm ±0.05μm), thickness of pulmonary arterioles increased (73.78μm ±7.61μm vs.40.16μm ±2.17μm), the differences were statistically significant ( P <0.05).Compared with the MCT model group , all indexes of EPCs treatment group were significantly improved , pulmonary arterial pressure was (22.78 ± 2.71) mmHg, right ventricular hypertrophy index was (0.41 ±0.05),pulmonary artery wall thickness was (16.55 ±3.15)μm, pulmonary artery thickness was (53.50 ±3.77) μm,the difference were statistically significant ( P <0.05).Conclusion The transplantation of peripheral blood endothelial progenitor cells could affect pulmonary artery hypertension in rabbit model , repair the lung vascular system damage to a certain extent ,improve the pulmonary arterial hypertension .关键词
外周内皮祖细胞/移植/肺动脉高压/右心肥大指数/新西兰兔Key words
Peripheral endothelial progenitor cells/Transplantation/Pulmonary hypertension/Right ventricular hy-pertrophy index/New Zealand rabbits引用本文复制引用
杨志瑜,戚国庆,夏岳,张燕,张庆文,韩巍,尤扬,魏立业..外周血内皮祖细胞移植对肺动脉高压兔模型肺动脉压力及其结构的影响[J].疑难病杂志,2014,(5):509-512,4.基金项目
河北省卫生厅指令计划支持科研课题 （）
