摘要
Abstract
Objective To investigate the correlation among the hepatic lipase ( HL) gene promoter 514C/T polymor-phism, type 2 diabetes (DM) lipid metabolism and insulin resistance (IR).Methods Application the restriction fragment length polymorphism analysis of polymerase chain reaction ( PCR-RFLP) combined with DNA sequencing technology , 284 ca-ses of type 2 DM patients (DM group) and normal glucose tolerance in 197 healthy subjects (group NC) were enrolled and underwent for HL-514 C/T polymorphism detection , and to observe the correlation between the gene polymorphism , serum lipid metabolism and IR.Results (1) The patients in the DM group with C/C and C/T+T/T genotypes frequencies were 39.2%and 60.8%, C and T allele frequencies were 64.5%and 35.5%, NC group were 32.7%, 67.3%and 63.2%, 36.8%, 2 groups had no significant difference ( P >0.05).(2) The patients with C/T+T/T genotype three triglyceride level of DM group was (2.93 ±1.37) mmol/L, C/C genotype were (2.07 ±1.82) mmol/L, there was significant difference between the 2 groups ( P <0.05), the difference of rest indices were not statistically significant ( P >0.05).(3)The differences of rele-vant indices of C/T+T/T genotype and C/C genotype patients'IR were not significantly ( P >0.05).(4) Logistic regression analysis showed that , DM group's HL-514 C/T gene polymorphisms , BMI were positively correlated with TG anomalies ( OR=3.042, 95%CI 1.265-7.317;OR=2.600, 95%CI 1.520-4.447, P <0.05).Conclusion The HL-514C/T gene poly-morphism may had negative correlation with type 2 DM, as well with IR in type 2 DM, while the HL-514T allele may be a risk factor for patients with type 2 DM had high levels of TG .关键词
肝脂酶基因/糖尿病/血脂代谢/胰岛素抵抗/分子遗传学Key words
Hepatic lipase gene/Diabetes/Lipid metabolism/Insulin resistance/Molecular genetics
