Abstract
Objective To observe the effect of perindopril on pulmonary function and lung tissue inflammatory factor in chronic obstructive pulmonary disease ( COPD) rats. Methods 30 male SD rats were randomly divided into 3 groups:group A ( normal control group) , group B ( LPS group) , group C ( ACEI+LPS group) , 10 rats in each group. Using the 2 times tracheal instillation of lipopolysaccharide ( LPS) method and smoking to establish the rat model of COPD, detect rat pul-monary function and serum and lung homogenate's IL-8, TNF-αcontent, then to observe the effect of perindopril on rat pulmo-nary function and inflammatory factor. Results (1) Respiratory function in rats:group A, group B and C group's VE values (ml/min), were 180.50 ±23.58, 108.50 ±33.53 and 140.90 ±33.10; PEP value (ml/s) were 30.20 ±1.67, 20.30 ± 2. 78 and 25. 77 ± 3. 45; the value of FEV0. 3 ( ml) were 4. 91 ± 0. 30, 4. 01 ± 0. 30 and 4. 55 ± 0. 18 ; compared with A group, group B and C 's VE values were decreased by 40%, 22%, PEP value decreased by 33%, 15% respectively, the FEV0. 3 decreased by 18%, 7% respectively. Compared among the 3 groups, the difference was statistically significant (P<0. 05). (2) Serum IL-8 content (ng/L) in rat, groups A, B, C were 211. 69 ± 13. 40, 386. 10 ± 26. 52, 324. 58 ± 25. 49;lung homogenate ingroup A, B, C were 348. 96 ± 4. 78, 439. 28 ± 15. 15, 394. 00 ± 9. 78, compared among the 3 groups, the differences were statistically significant ( P <0. 05). The serum content of TNF-α in rat, group A, B, C were 42. 28 ± 2. 55 and 102. 86 ± 5. 99, 68. 90 ± 3. 20;lung tissue homogenate in group A, B, C were 131. 44 ± 7. 90,201. 21 ± 4. 98, 154. 34 ± 4. 73, compared among the 3 groups, the difference was statistically significant ( P <0. 05). Conclusion It demonstrated that the perindopril could significantly improve lung function of COPD rats and its mechanism may be realized through the down-regulation serum IL-8, TNF-α content and lung tissue homogenate in COPD rats.关键词
慢性阻塞性肺疾病/白介素-8/肿瘤坏死因子-α/培哚普利/脂多糖/大鼠Key words
Chronic obstructive pulmonary disease/Interleukin-8/Tumor necrosis factor alpha/Perindopril/Lipopo-lysaccharide/Rats
