摘要
Abstract
Objective To analyze the behavioral characteristics in the different types of cerebral ischemia in rats aftermiddle cerebral artery occlusion (MCAO) method by inserting a nylon filament and the relationships between behavioral performanceand the size of infarct volume.Methods 101 male SD rats were used in this study.The MCA were blocked by a nylonfilament and reperfusion after 100 minutes.To determine the site and the volume of infarction, MRI scan were performedin each rat 24 hours after the surgery.The rats were divided to three groups that cortex basal ganglia infarction group( CIG, n =51), sub cortical infarction group (SIG, n =24) and non-infarction group (NIG, n =26) according to the MRI scan results.The behavioral tests, including forelimb use asymmetry, limb placement tests, and neurological severity score (NSS)were used to evaluate the neurologic deficits at the second day after the MCAO surgery.Results In the forelimb use asymmetrytest, there was difference between CIG and NIG ( q =3.72, P <0.05), and this difference also existence between SIGand NIG ( q =3.28,P <0.05), but between CIG and SIG showed no difference ( q =0.44,P >0.05).In the limb placementtests and the NSS tests, there was significant difference between CIG and NIG ( q =12.02, q =11.98,P <0.01), alsobetween CIG and SIG ( q =10.28, q =11.48, P <0.01), at this time there was no significant difference between SIGand NIG ( q =1.74, q =0.50, P >0.05).And in the CIG and SIG, there was no significant difference between the volumeof infarction and the results of the three behavioral tests ( r =-0.087,r =0.243,r =-0.226, r =0.279,r =0.010, r =0.110, P >0.05).Conclusion The MCAO method can appear different infarction types, and the behavioral characteristicsare different in these types.This difference occurs may not be associated with the size of the infarct volume.关键词
大脑中动脉阻塞模型/大鼠/类型/行为学/脑梗死体积Key words
Middle cerebral artery occlusion/Rats/Types/Behavioral tests/Infarct volume
