亚热带植物科学Issue(3)：202-205,4.DOI:10.3969/j.issn.1009-7791.2014.03.004
大孔树脂分离纯化有柄石韦总黄酮的工艺优化
Optimization of Separation and Purification Procedure of Total Flavonoids in Pyrrosia petiolosa by Macroporous Adsorption Resin
摘要
Abstract
To select the optimum purifying process of total flavonoids in Pyrrosia petiolosa, orthogonal experiment was designed with the desorption rate of total flavonoids and the content of total flavonoids as indexes. Theresults showed that the best separation conditions were determined as: sample concentration 0.5 mg·mL-1at pH 6, eluent concentration 50%, elution rate 2 mL·min-1by D-101macroporous resin.The concentration of total flavonoids in the filtrate could reach 76.40%, and the method was stable and feasible, and the result was reliable.关键词
大孔吸附树脂/有柄石韦/总黄酮/分离纯化Key words
macroreticular resin/Pyrrosia petiolosa/total flavonoids/separation and purification分类
医药卫生引用本文复制引用
刘静,程丹丹,辛晓伟,高德民,张宏萌..大孔树脂分离纯化有柄石韦总黄酮的工艺优化[J].亚热带植物科学,2014,(3):202-205,4.基金项目
山东中医药大学2013年SRT 项目 （）
