烟台大学学报（自然科学与工程版）Issue(3)：179-185,7.DOI:10.13951/j.cnki.371213/n.2015.03.006
海生红藻多管藻中藻胆蛋白的分离纯化
Isolation and Purification of Phycobiliproteins from Marine Red Alga Polysiphonia urceolata
摘要
Abstract
R-phycerythrin ( R-PE) , R-phycocyanin ( R-PC) and allophycocyanin ( AP) are isolated from the extrac-ted phycobiliprotein solution of Polysiphonia urceolata rich in phycoerythrin using gel filtration through Middle Fine Sephadex G-150 (MFG-150), Fine Sephadex G-150(FG-150) and Sephacryl S-300(S-300). The obtained fractions of R-PE, R-PC and AP are further purified using ion-exchange chromatography through DEAE Sepharose-Fast Flow. An optimized technical procedure for simultaneous preparation of the three phycobiliprotein types from the extracted phycobiliproteins of Polysiphonia urceolata is established based on the present experiments.关键词
层析/聚丙烯酰胺凝胶电泳/多管藻/藻红蛋白/藻蓝蛋白/别藻蓝蛋白Key words
chromatography/polyacrylamide gel electrophoresis/Polysiphonia urceolata Grev/phycoerythrin/phy-cocyanin/allophycocyanin分类
生物科学引用本文复制引用
郭凝,王玉,赵明日,龚雪琴,孙力..海生红藻多管藻中藻胆蛋白的分离纯化[J].烟台大学学报（自然科学与工程版）,2015,(3):179-185,7.基金项目
国家自然科学基金资助项目(30571720,40976083) （30571720,40976083）
