医学信息Issue(14)：56-57,2.
AKT在Ghrelin抑制结肠癌细胞凋亡中的作用
Ghrelin Inhibit Cell Apoptosis in Human Colorectal Cancer Cell Line RKO Via AKT Pathway
摘要
Abstract
Objective To investigate the anti-apoptotic ef ect and the mechanism of Ghrelin in human colorectal cancer cellline RKO in vitro. Methods After human colorectal cancer cellline RKO was 5-FU treatment to induce apoptosis and simultaneously treated with Ghrelin in the presence or absence of wortmannin, a highly specific inhibitor of AKT. The cellular proliferation was detected by cellCounting Kit-8 (CCK-8) assay. The morphological changes of RKO cells were observed under the fluorescence microscopy after DAPI staining. Western blotting was used to detect the expression of p-AKT in RKO cells. Results Ghrelin prevented apoptosis in RKO cells induced by 5-FU. However, wortmannin abolished Ghrelin' s cytoprotective activity after 5-FU treatment. Ghrelin treatment markedly increased AKT activation in RKO cells, and this was completely suppressed by coincubation with wortmannin. Conclusion Ghrelin may act as a survival factor in RKO cells through the activation of AKT, and Ghrelin might become a potential therapeutic target of colorectal cancer.关键词
结肠癌/Ghrelin/AKTKey words
Colorectal cancer/Ghrelin/AKT引用本文复制引用
唐珍,刘岸,唐敏..AKT在Ghrelin抑制结肠癌细胞凋亡中的作用[J].医学信息,2014,(14):56-57,2.基金项目
岳阳市科技计划项目 （）
