医学信息Issue(15)：227-228,2.
脱细胞同种异体神经移植物组织相容性初探
Acellular Allogenic Nerve Graft Tissue Biocompatibility Study
孙慧哲 1曾亮 1田伟 1王效杰 1佟晓杰2
作者信息
- 1. 沈阳医学院人体解剖学教研室，辽宁 沈阳 110034
- 2. 中国医科大学人体解剖学教研室，辽宁 沈阳 110001
- 折叠
摘要
Abstract
Objective To explore histocompatibility of acellular rat sciatic nerve. Methods Prepared acellular rat sciatic nerve(ARSN) with hypotonic acellular treatment. Histological observation and examined by immunohischemical method. Ananlyzed histocompatibility after implantation in muscle respectively. Results ①ARSN reserved basement membrane tube of SC (Schwann cells) and ECM (extracellular matrix) of epineurium and perineurium, while axis-cylinder, myelin sheath and cellstructure of epineurium and perineurium were cleared away. ②Histological observation of ARSN after implantation in muscle. After HE staining showed: on the 4th day, epineurium left in ARSN group was absorbed obviously. Also, a ring structure formed of new-born granulation tissue surrounded ARSN. On 7th day of implantation, epineurium left in ARSN was absorbed completely and granulation tissue tended to be mature. And we saw no lymphocyte. ③Examination of laminin (LN) and fibronectin (FN) by immunohistochemical method, We could see that basement membrane was yel ow or brown showing positive reaction. Conclusion The cytoskeletal of acl ular peripheral ECM we prepared reserved important components of ECM of nerve tissue which might promote nerve regeneration, LN and FN. They had good histocompatibility and would be degraded with time flies.关键词
神经组织工程/脱细胞神经异体移植物/神经再生/大鼠Key words
Nerve tissue-engineering/Acellular peripheral nerve al ograft/Nerve regeneration/Rat引用本文复制引用
孙慧哲,曾亮,田伟,王效杰,佟晓杰..脱细胞同种异体神经移植物组织相容性初探[J].医学信息,2014,(15):227-228,2.
