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不同产地灵芝孢子粉中多糖、总三萜皂苷含量测定

邓桂球 苏振宁 张健润 吴茂勇 吴依娜

医学信息Issue(19):48-49,2.
医学信息Issue(19):48-49,2.

不同产地灵芝孢子粉中多糖、总三萜皂苷含量测定

Determination of the Content of Different Origin of Ganoderma Lucidum Polysaccharide, Total Three Terpene Saponins

邓桂球 1苏振宁 2张健润 2吴茂勇 2吴依娜2

作者信息

  • 1. 广州市白云区第一人民医院,广东 广州 510405
  • 2. 广州中医药大学中药学院,广东 广州 510006
  • 折叠

摘要

Abstract

Objective To establish a method for content determination of total polysaccharides and triterpenoid saponin in the GLS.Methods UV Spectrophotometry,phenol-sulfuric acid;vanillin-glacial acetic acid-sulphuric acid-perchloric acid mixture at the wavelength of 492nm and 555nm absorbance.Results The linear range of total ginseng polysaccharides was 0.0100~0.0300mg(=0.9982);0.025~0.125mg(=0.9996).The recovery rate of the study was 101.17%、100.74%,RSD=1.84%、1.29%.Conclusion The results of simulation indicate that this method was accurate,reliable and reproducible.It was suggested that this method can be used to determine the content of total polysaccharides and triterpenoid saponin in the GLS.

关键词

灵芝孢子粉/灵芝多糖/总三萜皂苷/含量测定/紫外分光光度法

Key words

GLS/Polysaccharides/Triterpenoid saponin/Determination/Ultraviolet spectrophotometry

引用本文复制引用

邓桂球,苏振宁,张健润,吴茂勇,吴依娜..不同产地灵芝孢子粉中多糖、总三萜皂苷含量测定[J].医学信息,2014,(19):48-49,2.

基金项目

广东省科技计划(2012A030100003);白云区科技支撑计划 ()

医学信息

1006-1959

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