医学信息Issue(19)：88-89,2.
深低温冷冻法与酶洗法制备兔组织工程同种异体气管支架研究
Cryoapplication Methods and Detergent-Enzymatic of the Rabbit tissue Engineering Allogeneic Tracheal Stent
李建忠 1吴凡 2汪健 2张志培 2闫小龙 2梁兵 1高峰 1马朝晖 1李小飞2
作者信息
- 1. 中国人民解放军第211医院心胸外科，辽宁 沈阳 150080
- 2. 第四军医大学唐都医院胸外科，陕西 西安 710038
- 折叠
摘要
Abstract
Objective This study mainly focused on finding out the optimal method to obtaine rabbit histological engineering tracheal matrix between cryoapplication and detergent-enzymatic. Methods 18 adult rabbits were divided into 3 groups (each n =6), and group A were not treated as control group,groupB was treated with the method of cryopreservation,group C was treated with detergent-enzymatic method. HE dyes and scanning electron microscopy were used to observe the morphology and ultrastructure of the treated tracheal matrices. The biomechanical properties including maximum tensile force, rupture force and aberration rate of the treated tracheal matrices were measured. Results There were a lot of epithelial cells in the tracheas of group A and some epithelial cells in the tracheas of group B.But the tracheas of group C could not be seen complete epithelial cells. Under scanning electron microscopy,there were abundant extracellular matrix and col agen fiber in group A, B and C. Final y, no statistical dif erences were found in the maximum tensile force, rupture force and aberration rate of al groups. Conclusion The method of cryopreservation could retain abundant extracellular matrix and col agen fiber for tracheal stent. Howevr,this way can not remove the antigen. The method of enzymatic, by which the antigen can be removed, and extracellular matrix,but it can not retain a lot of extracellular matrix.关键词
深低温冷冻法/酶洗法/组织工程气管支架/细胞外基质Key words
Deep freezing method/Enzyme method/Tissue engineering tracheal stent/Extracellular matrix引用本文复制引用
李建忠,吴凡,汪健,张志培,闫小龙,梁兵,高峰,马朝晖,李小飞..深低温冷冻法与酶洗法制备兔组织工程同种异体气管支架研究[J].医学信息,2014,(19):88-89,2.
