医学信息Issue(21)：58-59,2.
负载HER-2的树突状细胞诱导T细胞对骨肉瘤杀伤活性的研究
Anti-cancer Effect of T Lymphocyte Induced by Dendritic Cells Plused with HER-2 Antigen to Osteosarcoma
丁利伟 1刘林 2许铁2
作者信息
- 1. 徐州医学院研究生学院，江苏 徐州 221004
- 2. 徐州医学院急救与救援医学系驭徐州医学院附属医院急救中心，江苏 徐州 221002
- 折叠
摘要
Abstract
Objective To investigate anti-cancer immunity of the T lymphocyte activated by dendritic cells loading of HER-2 antigen against osteosarcoma cell lines U2-OS. Methods Using HER-2 antigen peptide E75369-377 (HER-2 group), PBS (PBS group) loaded PBMC-derived dendritic cells respectively, then cultured with T lymphocytes for 7 days. Detected the expression of T cells' perforin, and compared the kil ing activity of T cells in each group to U2-OS cells by LDH release assay. Results T cells in HER-2 group expressed perforin higher than PBS group (P <0.01); The kil ing rate of T cellto U2-OS cells is higher in HER-2 group than in PBS group detected by LDH release assay ( P<0.01). Conclusion The U2-OS whole tumor antigen loading DC to stimulate T lymphocytes activation is bet er than synthetic peptide HER-2 antigen. With HER-2 antigen peptide E75369-377 loading dendritic cells can induce T cells to improve the ability of kil ing osteosarcoma celllines U2-OS .关键词
树突状细胞/HER-2/骨肉瘤/免疫治疗Key words
Dendritic cells/HER-2/Osteosarcoma/Immunological therapy引用本文复制引用
丁利伟,刘林,许铁..负载HER-2的树突状细胞诱导T细胞对骨肉瘤杀伤活性的研究[J].医学信息,2014,(21):58-59,2.
