医学信息Issue(38)：20-21,2.
pCDNA 3.1/EC-SOD真核载体的构建及在巨噬细胞中的表达
Construction of Eukaryotic Expression Vector pCDNA 3.1/EC-SOD and Its Expression in Macrophage Cells
摘要
Abstract
To investigate the ef ect of EC-SOD in development of atherosclerosis (As), we recombination vector of pCDNA 3.1/EC-SOD in vitro and to observe its expression in monocyte-derived macrophages. METHODS EC-SOD fragment were cloned and linked to plasmid pCDNA 3.1 in vitro. After plasmid polymerase chain reaction (PCR), Restriction enzyme digestion and sequencing to confirm the vector of EC-SOD was successful y constructed, with the help of Lipofectamine 2000 we transfected the vector to macrophages, and we detected the transfection ef iciency with fluorescence. CONCLUSION The vector of EC-SOD was successful y constructed and its working in macrophages, this work was a great base for study the role of EC-SOD in development of As.关键词
EC-SOD/THP-1单核源性巨噬细胞Key words
ECSOD/THP-1 monocyte-derived macrophage cel s引用本文复制引用
张晓娟,贾月霞,姜怡邓,张辉,孔繁琪,王艳华,刘现梅,赵丽,和杨杨,朱光荣,王楠..pCDNA 3.1/EC-SOD真核载体的构建及在巨噬细胞中的表达[J].医学信息,2014,(38):20-21,2.基金项目
国家自然科学基金(81260105 （）
81260063 （）
81160044) （）
宁夏自治区科技厅科技攻关项目20100820，[2012]17号 （）
