摘要
Abstract
Objective To study the heparin and EDTA anticoagulant blood for blood ammonia detection results whether there are dif erences. Methods According to blood ammonia reference range selected cases into Ⅰ, II, Ⅲ three groups,each group of 30 patients, 90 patients. Respectively adopt its venous blood about 3 ml, inject heparin anticoagulation, EDTA anticoagulation two dif erent vacuum acquisition tube, the centrifugal separation namely after extraction plasma or serum with dry chemical method for blood ammonia value determination and calculation mean and dif erence, car ies on the statistical analysis, and compared between the two groups have statistical significance dif erence mean, the t test. ResultsⅠgroup heparin anticoagulation group value was 22.23±6.19(μmol/L), EDTA anticoagulation group value was 40.5±15.68 (μ mol/L), two groups of compared = 5.94, < 0.01; II group heparin anticoagulation group value was 50.79±13.68 (μ mol/L), EDTA anticoagulation group value was 74.35±20.9 (μ mol/L), two groups of phase comparison, = 5.17, < 0.01; Ⅲ group heparin anticoagulation group value was 121.59±24.72 (μ mol/L),EDTA anticoagulation group value was 156.83±21.82 (μ mol/L), two groups of phase comparison, = 5.85, < 0.01. Conclusion Dif erent blood col ection tube to dry chemical method blood ammonia determination results have a certain influence, heparin anticoagulation group of the determination results below EDTA anticoagulation group, there is significantly statistical dif erence ( < 0.01). Because of this experiment is influenced by environmental temperature, specimen placed time and so on factors, and the stability of the heparin higher. Therefore, in order to improve the veracity of test results, it is suggested that clinical blood ammonia determination using heparin anticoagulation vacuum tube specimen col ection.关键词
血氨测定/抗凝剂/肝素/EDTAKey words
Ammonia determination/Anticoagulant/Heparin/EDTA
