摘要
Abstract
Objective Research and Analysis of hepatoma cel lines by doxorubicin treated with sorafenib and mechanism of inhibition of HepG2.Methods According to drug use into sorafenib group (Group A),doxorubicin group (Group B),doxorubicin combined with sorafenib group (Group C);and acts on the liver cel line HepG2 cel s,respectively.Analysis and comparison of the three groups and inhibition of cel proliferation and apoptosis and so on.Results Three groups can ef ectively inhibit the proliferation of HepG2 cel s in a dose-dependent manner;but Group C has a synergistic ef ect( <0.05).A,B can make HepG2 cel cycle ar est in G0-G1 phase;but Group C G0/G1 phase of the cel ratio was significantly lower than the A and B groups;was significantly higher than that of B and S groups ( <0.05).Three groups can induce apoptosis in HepG2 cel s,but Group C is more significant ( <0.05);B can ef ectively inhibit Survivin mRNA expression in HepG2 cel apoptosis induced cel s. Conclusion Doxorubicin combined with sorafenib for hepatocel ular carcinoma cel line HepG2 has good synergistic ef ect,but can also accelerate cel proliferation,thereby restrain the growth ef ect of tumor cel s.Therefore,further clinical studies to bet er provide reference for clinical treatment of liver cancer,thereby improving patient outcomes.关键词
阿霉素/索拉菲尼/肝癌细胞株/HepG2/抑制Key words
Adriamycin/Sola Feeney/Hepatocel ular carcinoma/HepG2/Inhibition
