医药导报Issue(5)：565-568,4.DOI:10.3870/yydb.2014.05.004
丹红注射液对SMMC-7721肝癌细胞的促凋亡作用
Pro-Apoptosis Effects of Danhong Injection on the SMMC-7721 Hepatoma Cells
仲蕾 1徐立平 1钱先中 1金慧静 1陈超 1仵利军1
作者信息
- 1. 解放军第100医院药械科，苏州 215007
- 折叠
摘要
Abstract
Objective To investigate the effects of danhong injection on the apoptosis of SMMC-7721 heptoma cells.Methods The hepatocellular carcinoma cell line SMMC-7721 was incubated with different concentrations(0,2,4,8 μL·mL-1) of danhong injection, and cell morphology was studied under the microscope. The apoptosis index was detected by flow cytometry at 0,6,12,24 h after cultured with 2 μL·mL-1danhong injection. Cell proliferation was measured by MTT assay;Gene expressions of p53,Bax and Bcl-2 were detected by RT-PCR at different drug concentrations. Results The morphology of the hepatoma cells changed obviously,and the apoptosis index increased by danhong injection in a dose-dependant manner. The danhong injection decreased gene expressions of Bcl-2,and obviously increased p53 and Bax. Conclusion Danhong injection can dose-and time-dependently promote apoptosis of SMMC-7721 cells.关键词
丹红注射液/SMMC-7721肝癌细胞/凋亡Key words
Danhong injection/SMMC-7721 hepatoma cells/Apoptosis分类
医药卫生引用本文复制引用
仲蕾,徐立平,钱先中,金慧静,陈超,仵利军..丹红注射液对SMMC-7721肝癌细胞的促凋亡作用[J].医药导报,2014,(5):565-568,4.
