医药导报Issue(5)：650-652,3.DOI:10.3870/yydb.2014.05.031
3-酰胺基-吲哚类衍生物及其有关物质含量测定
Determination of Contents 3-amide-indole Derivative and Related Substances
摘要
Abstract
Objective To establish a RP-HPLC method for the determination of 3-amide-indole derivative and its related substances. Methods Diamonsil C18(250 mmí4. 6 mm,5 μm) column was adopted. The mobile phase consisted of a mixture of methanol-acetonitrile-water(431245) at the flow rate of 1. 0 mL·min-1 . The wavelength for ultraviolet detection was 224 nm. The injection volume was 20 μL and the column temperature was room temperature. Results 3-amide-indole derivative and related substances could be well separated. The linearity of the 3-amide-indole derivative curve was well correlated (r=0. 999 7) within the range of 0. 04-0. 16 mg·mL-1. The RSD was 0. 52%with good repeatability. The detection limit was 2. 65 ng. Conclusion The method is accurate,reliable,sensitive and specific,which could be used for the determination of 3-amide-indolederivative and related substances.关键词
3-酰胺基-吲哚类衍生物/色谱法,高效液相/含量测定Key words
3-amide-indole derivative/Chromatography, high performance liquid/Contents determination分类
医药卫生引用本文复制引用
申冬妮,杨建云,肖炳坤,黄荣清..3-酰胺基-吲哚类衍生物及其有关物质含量测定[J].医药导报,2014,(5):650-652,3.基金项目
国家自然科学基金项目(81072613) （81072613）
“十二五”国家科技重大专项“重大新药创制”综合性新药研究开发技术大平台(2012ZX09301003-001-010) （2012ZX09301003-001-010）
