医药导报Issue(6)：723-726,4.DOI:10.3870/yydb.2014.06.008
吡非尼酮对大鼠肝细胞色素P450酶活性的影响
Effect of Pirfenidone on Activity of Hepatic Cytochrome P450 in Rats
吴际 1陈汇 2吴健鸿 3周顺长 4曾繁典2
作者信息
- 1. 武汉科技大学城市学院，武汉 430083
- 2. 华中科技大学同济医学院药理教研室，武汉 430030
- 3. 武汉药品医疗器械检验所，武汉 430075
- 4. 华中科技大学同济医学院动物实验中心，武汉 430030
- 折叠
摘要
Abstract
Objective To study the effects of pirfenidone on total enzyme and isoenzyme of liver microsomal cytochrome P450 in rats. Methods The activites of liver microsomal dimethyl nitrosamine N-demethylase( NDMA )and erythromycin demethylase( ERD ) were determined. Fifty-six SD rats were randomly divided into six groups,in which they received CMC,dexamethasone 100 mg·kg-1 ,ketoconazole 40 mg·kg-1 ,pirfenidone 25,50,100 mg·kg-1 ,respectively. After administration for 6 and 12 days,livers were prepared liver microsome,the concentration of proteinum in microsome and shade selection to plasma samples were determined by spectrophotometer. Results After administration of pirfenidone for 6 days, cytochrome P450 was significantly increased in 50 and 100 mg·kg-1 pirfenidone groups,as compared with solvent control group (1%sodium carboxymethylcellulose)(P﹤0. 01). After administration of pirfenidone for 6 and 12 days,NDMA of liver microsome was not changed significantly(P﹥0. 05). ERD of liver microsome was significantly increased in 100 mg·kg-1 pirfenidone group after administration for 12 days(P﹤0. 01). Conclusion Pirfenidone can induce P450 and ERD activities in a dose-and time-dependent manner.关键词
吡非尼酮/细胞色素P450酶/酶活性Key words
Pirfenidone/Cytochrome P450/Enzyme activity分类
医药卫生引用本文复制引用
吴际,陈汇,吴健鸿,周顺长,曾繁典..吡非尼酮对大鼠肝细胞色素P450酶活性的影响[J].医药导报,2014,(6):723-726,4.
