医药导报Issue(8)：988-991,4.DOI:10.3870/yydb.2014.08.003
复方鹿角霜对胶原诱导性关节炎模型小鼠抗炎作用研究
Anti-inflammatory Effect of Compound Cornu Cervi Degelatinatum on Collagen-induced Arthritis Model of Mice
摘要
Abstract
Objective To investigate the effects of compound cornu cervi degelatinatum on the expression of related inflammatory cytokines and pathological changes of the synovium in mice with collagen-induced arthritis (CIA). MethodsThe CIA model of Balb/c mice was established. The mice were randomly divided into 5 groups: blank control group, model control group, low-dose group ( C1 ) , high-dose group ( C2 ) and glucosidorum tripterygll totorum group D. After the first immunization in mice, we observed the general condition and lesions of the fore and hind jaws. After the second immunization, the mice in groups C1 and C2 were orally administered with compound cornu cervi degelatinatum at the doses of 2. 5 and 5 g·kg-1 body weight per day for 4 weeks, and meanwhile those in group D were administrated with glucosidorum tripterygll totorum at the dose of 13. 6 mg·kg-1 body weight per day. The serum of the mice was collected to detect the levels of TNF-αand IL-4 by ELISA. Ankle joints were harvested, and the pathological changes of synovial tissues were observed under light microscope. Results As compared with the model control group, the level of TNF-αin the treatment groups was significantly decreased, while the level of IL-4 was elevated in group C1. Histological pathology of ankle joints demonstrated that the synovium of the CIA mice were hyperplastic and the synovial tissues were markedly ameliorated in treatment groups. Conclusion The compound cornu cervi degelatinatum can relieve redness and swelling in mice with CIA. The mechanism may be related to the inhibition of pro-inflammatory cytokine TNF-α and regulation of anti-inflammatory cytokine IL-4.关键词
鹿角霜,复方/关节炎/胶原,Ⅱ型/细胞因子Key words
Cornu cervi degelatinatum,compound/Arthritis/Collagen, type II/Cytokine分类
医药卫生引用本文复制引用
陈腊霞,王燕燕,孙伟光,许鑫..复方鹿角霜对胶原诱导性关节炎模型小鼠抗炎作用研究[J].医药导报,2014,(8):988-991,4.基金项目
国家“重大新药创制”科技重大专项项目(2011ZX09102-006-05) （2011ZX09102-006-05）
