医药导报Issue(8)：1070-1072,3.DOI:10.3870/yydb.2014.08.025
高效液相色谱法同时测定肉豆蔻麸煨前后肉豆蔻木脂素和去氢二异丁香酚的含量
Determination of Content of Nutmeg Lignan and Dehydrodiisoeugenol in Nutmeg by HPLC
摘要
Abstract
Objective To develope a high performance liquid chromatograph ( HPLC ) method for simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after processing of nutmeg. Methods The column was Diamonsil C18(250 mmí 4. 6 mm, 5 μm). The mobile phase was methanol-water in a gradient elution mode. The UV detection wave length was 274 nm. The column temperature was 25℃ . The flow rate was 1. 0 mL·min-1 . Results Nutmeg lignan and dehydrodiisoeugenol had a good linear correlation in ranges of 10. 24-61. 44 μg·mL-1(r=0. 999 6) and 3. 0-18. 0 μg·mL-1 (r=0.999 8), respectively. The average recovery rates were 97. 94% (RSD=2. 17%) and 97. 11% (RSD=2. 17%). Conclusion The method is simple, accurate, reproducible, and can be used for the simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after the processing of nutmeg.关键词
肉豆蔻/肉豆蔻木脂素/去氢二异丁香酚/色谱法,高效液相Key words
Nutmeg/Nutmeg lignan/Dehydrodiisoeugenol/Chromatograph, high performance liquid分类
医药卫生引用本文复制引用
刘欢,陈剑锋,王静,袁子民..高效液相色谱法同时测定肉豆蔻麸煨前后肉豆蔻木脂素和去氢二异丁香酚的含量[J].医药导报,2014,(8):1070-1072,3.基金项目
国家自然科学基金资助项目(81274084) （81274084）
