医药导报Issue(9)：1129-1131,3.DOI:10.3870/yydb.2014.09.003
BQ123对大鼠肢体缺血-再灌注后肺损伤的影响
BQ123 Alleviates Lung Injury Following Limb Ischemia-Reperfusion in Rats
摘要
Abstract
Objective To observe BQ123 on lung tissue injury following ischemia-reperfusion of hind limbs in rats.Methods A total of 30 male SD rats were randomly divided into control,I-R,and BQ123 groups,with 10 rats in each group. After 4- hour of ischemia and 4-hour of reperfusion to the hind limbs, ET-1 was measured by radioimmunoassay, levels of myeloperoxidase (MPO) and malondialehyde (MDA) were examined by biochemical method,and the content of P-selectin was examined by ELISA. Immunohistochemical method was used to detect the expressions of Fas,Bcl-2 and Caspase-3 in lung tissues. Pulmonary apoptosis was examined by means of TdT-mediated dUTP nick end labeling ( TUNEL) . Results Compared with the control group,the levels of ET-1,MPO,MDA and P-selectin in lung tissues were all increased significantly in I-R group ( P<0. 01). The expressions of Fas,Bcl-2 and Caspase-3 were 0. 294±0. 003,0. 108±0. 005,and 0. 174±0. 003,significantly up-regulated in the I-R group. The apoptosis rate [(18. 83±2. 86)%] was significantly increased in the I-R group (P<0. 01). Compared with I-R group,the tissue contents of ET-1,MPO,MDA and P-selectin were significantly lowered in BQ123 group (P<0. 01). The expression levels of Fas (0. 115±0. 007) and Caspase-3 (0. 159±0. 006) were decreased,but the expression of Bcl-2 was increased (0. 128±0. 005). The apoptosis rate in BQ123 group was significantly lower [(10. 67±2. 16)%,P<0. 01].Conclusion BQ123 may have protective effects on lung tissue after limb ischemia-reperfusion in rats by means of improving neutrophil aggregation and reducing the expression of proteins related to cell apoptosis.关键词
摇 BQ123/缺血-再灌注/凋亡/内皮缩血管肽类Key words
BQ123/Ischemia-reperfusion/Apoptosis/Endothelis分类
医药卫生引用本文复制引用
刘燕,彭海兵,王建辉,王银环,张娜,李颖,朱丽艳,杨秀红,张连元..BQ123对大鼠肢体缺血-再灌注后肺损伤的影响[J].医药导报,2014,(9):1129-1131,3.基金项目
河北省科技厅研究与发展计划项目(12277793) （12277793）
唐山市科技局科研基金资助课题(12140209A-27) （12140209A-27）
