医药导报Issue(9)：1224-1226,3.DOI:10.3870/yydb.2014.09.032
高效液相色谱法同时测定补肾化瘀浸膏中淫羊藿苷及柚皮苷含量
Simultaneous Determination of Icariin and Naringin in Bushen Huayu Extract by HPLC
摘要
Abstract
Objective To establish a method for simultaneous determination of icariin and naringin content in bushen huayu extract by HPLC. Methods Reverse-phase high performance liquid chromatography ( RP-HPLC ) separation was performed one C18 column (4.6 mmí250 mm,5 μm).The mobile phase was acetonitrile containing 0.2%H4PO3(pH=2.9). Gradient elution with a flow rate of 0. 8 mL·min-1 was applied to achieve the separation. The detection wavelength was set at 269 nm,and the column temperature was 30℃. Results Icariin had a good linear range from 0. 3-3. 0μg (r=0. 999 9),the recovery rate was 105. 6%,and RSD was 0. 95%. Naringin was linear within the range of 0. 12-1. 20 μg (r=0. 999 9). The recovery rate was 94. 0%and RSD was 0. 52%. Conclusion The method is simple,stable,accurate,and reproducible,which can be used as the quality control standard for bushen huayue extract.关键词
补肾化瘀浸膏/柚皮苷/淫羊藿苷/色谱法,高效液相Key words
Bushen huayu extract/Icariin/Naringin/Chromatography, high performance liquid分类
医药卫生引用本文复制引用
欧阳露,夏勇,夏鹏,李洪亮,汪选斌..高效液相色谱法同时测定补肾化瘀浸膏中淫羊藿苷及柚皮苷含量[J].医药导报,2014,(9):1224-1226,3.基金项目
十堰市科学技术研究与开发项目(ZD2011023) （ZD2011023）
湖北省教育厅项目(B20082412) （B20082412）
湖北省2011协同创新中心基金资助项目(4) （4）
