医药导报Issue(10)：1291-1293,3.DOI:10.3870/yydb.2014.10.009
姜辣素对小鼠 B16细胞黑素合成的影响
Effects of Gingerol on Melanogenesis of B16 Melanoma Cells
摘要
Abstract
Objective To study the effects of the gingerol on the melanogenesis in melanoma B16 cells. Methods Melanoma cells were cultured with gingerol at 12. 5, 25. 0, 50. 0, 100. 0, 200. 0 μmol · L-1 and positive control drug hydroquinone,respectively,using Dulbecco's modified eagle's medium(DMEM) as the blank control group. The cell proliferation was measured by methyl thiazolyltet tetrazolium ( MTT) colorimetric assay. The tyrosinase activity and melanin content were measured by colorimetry assay. Results Gingerol at different concentrations had inhibitory effect on B16 cell proliferation compared with the blank control group ( P < 0. 05 or P < 0. 01), the inhibition rate being more than 48% at the dosage of 200. 0 μmol·L-1 . Tyrosinase activity was inhibited significantly compared with blank control group(P<0. 05 or P<0. 01),the inhibition rate being up to 50% at the dosage of 200 μmol·L-1 . Melanin content was also decreased at all levels of gingerol compared with blank control group(P<0. 05 or P<0. 01),but not in a dose-dependent manner. The inhibition rate of melanin content reached the plateau at gingerol levels greater than 25. 0 μmol · L-1 . Conclusion Gingerol can inhibit the cell proliferation,tyrosinase activity and decrease melanin synthesis in certain range of concentrations.关键词
姜辣素/黑素瘤细胞/增殖/酪氨酸酶/黑素合成Key words
Gingerol/Melanoma/Proliferation/Tyrosinase/Melanin分类
医药卫生引用本文复制引用
神芳丽,霍仕霞..姜辣素对小鼠 B16细胞黑素合成的影响[J].医药导报,2014,(10):1291-1293,3.基金项目
新疆维吾尔族自治区青年基金项目(2012211B59) （2012211B59）
