医药导报Issue(10)：1373-1375,3.DOI:10.3870/yydb.2014.10.032
液相色谱-质谱联用法检测壮阳类中成药中西地那非的含量
Quantitative Determination of Sildenafil in Aphrodisiacs by LC-MS
摘要
Abstract
Objective To establish a method for the quantitative determination of sildenafil in aphrodisiacs by liquid chromatography-mass spectrometry (LC-MS). Methods Chromatography was carried on a Waters BEH C18 column (50 mmí2. 1 mm,1. 7 μm) with the column temperature being 35 ℃ and 0. 02 mol·L-1 ammonium acetate water solution (containing 0. 1% acetic acid)-acetonitril (7030) as the mobile phase at a flow rate of 0. 2 mL·min-1 . Determination was performed by multiple reaction monitoring mode in two channels,475→100,475→283,using electrospray ionization in positive ion mode. Results Sildenafil had a good linear relationship in the range of 0. 1986-1. 986 ng with the linear regression equation being Y=27 750X+15 232 (r=0. 995 2). The average recovery was 96. 6% with RSD being 1. 8% (n=9). The limits of quantitation and detection were 0. 04 ng and 0. 01 ng,respectively. Conclusion The determination results of 10 batches of samples show that the method is sensitive and accurate and can be used as an appropriate technique to detect sildenafil in aphrodisiacs.关键词
西地那非/液相色谱-质谱联用/多反应监测Key words
Sildenafil/Liquid chromatography-mass spectrometry/Multiple reaction monitoring分类
医药卫生引用本文复制引用
邓银华,章为..液相色谱-质谱联用法检测壮阳类中成药中西地那非的含量[J].医药导报,2014,(10):1373-1375,3.基金项目
湖南省卫生厅资助项目(C2012-13) （C2012-13）
