医药导报Issue(11)：1411-1415,5.DOI:10.3870/yydb.2014.11.003
松生等总黄酮提取工艺及镇痛抗炎作用研究
Analgesia and Anti-inflammatory Effect of Total Flavonoids Extracted from Euphorbia prolifera
摘要
Abstract
Objective The study aimed to investigate the best extraction process and the analgesia and anti-inflammatory properties of total flavonoids from Euphorbia prolifera. Methods Through single factor experiment and orthogonal test,factors that affect the extraction yield of total flavonoids were studied,including the extraction time,solid to liquid ratio, ethanol concentration and extraction times. The mice models of ear edema induced by xylene and twisting induced by acetic acid were used to evaluate the analgesia and anti-inflammatory effects of different extractions from Euphorbia prolifera. Results Total flavonoids were extracted by the refluxing method,and the optimum conditions were extracting for 3 hours,solid to liquid ratio of 1:30,ethanol content of 60% in the solvent,and processed for 2 times. The highest extraction yield of total flavonoids from Euphorbia prolifera was 5. 63%. The ethanol,ethyl acetate and n-butanol extracts decreased twisting and ear swelling in mice. Conclusion The extraction for total flavonoids from Euphorbia prolifera is simple,efficient and reproducible. The ethanol,ethyl acetate and n-butanol extracts of total flavonoids have obvious analgesia and anti-inflammatory effects.关键词
松生等/总黄酮/回流提取/镇痛/抗炎Key words
Euphorbia prolifera/Total flavonoids/Reflux extraction/Analgesia/Anti-inflammation分类
医药卫生引用本文复制引用
金岩,黄山,赵磊,裴小娜..松生等总黄酮提取工艺及镇痛抗炎作用研究[J].医药导报,2014,(11):1411-1415,5.基金项目
国家自然科学基金资助项目（81360686）；甘肃省教育厅硕士生导师项目 （）
