医药导报Issue(11)：1416-1419,4.DOI:10.3870/yydb.2014.11.004
bFGF对成纤维细胞增殖和胶原及纤维连接蛋白的影响
Effects of Basic Fibroblast Growth Factor on Proliferation as well as Collagen and Fibronectin Expression on Fibroblasts
摘要
Abstract
Objective To explore the mechanism and effects of basic fibroblast growth factor( bFGF)on skin wound healing. Methods Fibroblasts( FB)were isolated from normal skin and hypertrophic scar and cultivated by direct adherence method. FB were then treated with different concentrations of bFGF(0,0. 1,1,10,100,1 000 ng·mL-1 )and cultivated with serum-free medium for 72 hours. The proliferation and apoptosis of FB in each group were detected by cell counting and trypan blue staining. Content and gene expression of typeⅠand type Ⅲ collagen and fibronectin were determined by ELISA and RT-PCR,respectively. Results bFGF promoted the proliferation of FB at low concentrations promoted apoptosis of FB at higher concentrations. The proliferation of FB from hypertrophic scar was slower than that from the normal skin. bFGF significantly inhibited type Ⅰ collagen production from hypertrophic scar FB but not from the normal skin. Moreover,bFGF up-regulated fibronectin expression in the normal fibroblasts,but not in the hypertrophic scar. No change in type Ⅲ collagen expression and production was observed in FB from either source. Conclusion bFGF has differential effects and mechanisms on FB of the normal skin and hypertrophic scar,suggesting that bFGF may play a role in early phase of skin wound healing and scar formation.关键词
碱性成纤维细胞因子/成纤维细胞/增生性瘢痕/创伤修复Key words
Basic fibroblast growth factor/Fibroblast/Hypertrophic scar/Wound healing分类
医药卫生引用本文复制引用
武艳,杨岚,张羽飞,袁晓环,初彦辉..bFGF对成纤维细胞增殖和胶原及纤维连接蛋白的影响[J].医药导报,2014,(11):1416-1419,4.基金项目
2014年黑龙江省教育厅青年学术骨干支持计划项目 （）
