医药导报Issue(12)：1570-1574,5.DOI:10.3870∕yydb.2014.12.008
rhGM_CSF及纳米银对深域度烫伤创面愈合过程血管化的影响
Effect of Recombinant Human Granulocyte∕macrophage Colony_stimulating Factor and Nano_silver on Neovascularization in Healing Process of Skin with Deep II Degree Burn
摘要
Abstract
Objective To obserVe the effect of recombinant human granulocyte∕macroPhage colony stimulating factor ( rhGM_CSF) and nano_silVer as treatment for skin with deeP II degree burn. Methods DeeP II degree burn Wistar rat model was established. The rats were randomly diVided into three grouPs,Petrolatum treatment grouP (grouP A,n=30),nano_silVer treatment grouP (grouP B,n=30),and rhGM_CSF treatment grouP (grouP C,n=30). The Pathological changes of wound of the three grouPs were obserVed 1,4,7,10,14 and 21 days after the treatment. The concentration of VEGF in serums was measured with ELISA. The leVels of HIF_1α mRNA exPression were detected by RT_PCR. Results On day 10, neoVascularization deVeloPed in grouPs A, B and C. Healing rate of the wound was the highest in grouP C, lowest in grouP A, with significant differences among the three grouPs on day 14 and day 21 (P<0. 05). VEGF leVel Peaked on day 14 in grouP A,and on day 10 in grouPs B and C. There were significant differences in VEGF leVel on day 1 between grouP A and grouPs B,C,on day 7 between grouP A and grouP C,on day 10,14 and 21 among the three grouPs (P<0. 05),but no significant differences on day 4 among the three grouPs. Conclusion rhGM_CSF and nano_silVer treatment can accelerate neoVascularization of wound,and rhGM_CSF is better than nano_silVer.关键词
重组人粒细胞_巨噬细胞集落刺激因子/纳米银/烫伤创面/血管内皮生长因子/缺血坏死因子Key words
Recombinant human granulocyte_macroPhage colony stimulating factor/Nano_silVer/Burn wound/Vascular endothelial growth factor/HyPoxia inducible factor分类
医药卫生引用本文复制引用
杨景哲,温海玲,耿琪瑛,陈凤平,冯欣姝..rhGM_CSF及纳米银对深域度烫伤创面愈合过程血管化的影响[J].医药导报,2014,(12):1570-1574,5.基金项目
河北省2013年医学科学研究重点课题计划指令性课题(20130024) （20130024）
承德市科学技术研究与发展计划项目(20122164) （20122164）
