医药导报Issue(3)：302-305,4.DOI:10.3870/yydb.2015.03.005
类叶升麻苷对谷氨酸损伤 PC12细胞的保护作用
Protective Effects of Acteoside on PC12 Cell Injury Induced by Glutamate
摘要
Abstract
Objective To investigate the effect of acteoside on injury PC12 cells induced by glutamate. Methods PC12 cells were assigned into normal control group, model control group, positive drug group and acteoside(AS) treated group. Every group was treated by 1. 5 mmol·L-1 glutamate for 24 hours except for the control group, and the injury was antagonized by 10 μmol·L-1 Vit E and acteoside at different concentration(15. 625, 31. 25, 62. 5, 125 and 250 μmol·L-1 ). Cell morphology was observed by inverted microscope, cell survival was determined with MTT, LDH activity was measured by enzyme label kit, the MDA content and SOD activity were measured by TBA kit and WST kit, and the ROS was measured by Elisa kit. Results Compared with the model control group, all doses of acteoside could significantly improve the PC12 cell morphology and survival (P<0. 05), inhibit LDH activity and production of MDA and ROS (P<0. 05), increase the activity of SOD (P<0. 05), except for the lowest dose group. Conclusion Acteoside has protective effects on PC12 cells injured by glutamate.关键词
类叶升麻苷/谷氨酸/PC12 细胞/损伤/保护作用Key words
Acteoside/Glutamate/PC12 cell/Injury/Protective effect分类
医药卫生引用本文复制引用
彭晓明,霍仕霞,高莉,何燕,闫明..类叶升麻苷对谷氨酸损伤 PC12细胞的保护作用[J].医药导报,2015,(3):302-305,4.基金项目
新疆维吾尔自治区科研机构创新发展基金项目(2012015) （2012015）
新疆维吾尔自治区公益性科研院所基金项目(KY2013103) （KY2013103）
