医药导报Issue(5)：565-569,5.DOI:10.3870/yydb.2015.05.001
法舒地尔对结肠炎模型大鼠的治疗作用
Protective Effect of Fasudil on Inflammatory Bowel Disease of Rats
摘要
Abstract
Objective To investigate the treatment effect of fasudilon inflammatory bowel disease ( IBD) in rats. IBD was induced by using an enema of trinitro-benzene-sulfonic acid ( TNBS ) . Methods A total of 30 female SD rats were randomly divided into normal control group (0. 9% sodium chloride for induction and 0. 9% sodium chloride for treatment), model control group (TNBS for induction and 0. 9% sodium chloride for treatment), and fasudil group (TNBS for induction and fasudil 4. 5 mg·kg-1 for treatment). The disease activity index(DAI) was estimated 14 days later. The MPO activity, TNF-αcontent and IL-1β level in the colon were investigated, while Rho kinase expression was detected by Western blot. The pathological changes were detected by HE staining. Results The DAI of the normal control, model control, and fasudil groups were:(0. 00±0. 00),(7. 76±1. 32),and (3. 20±0. 98), respectively. MPO activity was (59. 32±9. 08),(96. 65±16. 57),and (69. 58±11. 40) U·g-1, respectively. TNF-α was (0. 15±0. 11),(0. 28±0. 22),and (0. 20±0. 62) ng·mL-1, respectively. IL-1β content was (0. 04±0. 01),(0. 08±0. 02),and (0. 06±0. 02) ng·mL-1, respectively. Rho kinase expression was 0. 713± 0. 170,1. 083±0. 210,and 0. 907±0. 260, respectively. Conclusion Fasudil excerts protective effects against IBD in rats by lowering the expression of Rho kinase and attenuating the release of inflammatory mediators, suggesting that Rho kinase could be a new therapeutic target for IBD.关键词
法舒地尔/肠病,炎症性/大鼠Key words
Fasudil/Bowel disease, inflammatory/Rat分类
医药卫生引用本文复制引用
望运玲,岳源,武双婵,丁虹..法舒地尔对结肠炎模型大鼠的治疗作用[J].医药导报,2015,(5):565-569,5.基金项目
国家自然科学基金资助项目(81273523) （81273523）
