医药导报Issue(5)：655-657,3.DOI:10.3870/yydb.2015.05.023
HPLC-DAD法检测染红中药中20种非法添加色素
Simultaneous Determination of 20 Pigments in Illegally Dyed Chinese Herbs by HPLC-DAD
摘要
Abstract
Objective To develop a HPLC-DAD method for the simultaneous determination of 20 synthetic pigments in dyed Traditional Chinese Medicine. Methods The samples were extracted with ultrasonic by 70% methanol, separated on a Agilent TC-C18 column ( 4. 6 mmí250 mm, 5 μm ) by gradient elution using a mobile phase made up of methanol and 0. 02 mol·L-1 ammonium acetate, and detected at 210-650 nm. The retention times and ultraviolet absorption spectrums were used to text and verify. Results The twenty red and orange synthetic pigments were separated and detected. OrangeIIsodium salt and 808 scarlet were detected in some batch of Croci stigma and Cinnabaris. Conclusion The method is simple, rapid, accurate, repeatable for determining twenty synthetic pigments in dyed Traditional Chinese Medicine.关键词
中药,染色/检测器,高效液相色谱-二极管阵列/色素检测/色素,人工合成/朱砂/酸性橙域Key words
Chinese herbs, dyed/Detection, high performance liquid chromatography-diode array/Detection of pigment/Pigment, synthetic/Cinnabaris/Orange II sodium salt分类
医药卫生引用本文复制引用
李启艳,朱日然,孙萍,姜国萍..HPLC-DAD法检测染红中药中20种非法添加色素[J].医药导报,2015,(5):655-657,3.基金项目
山东省中医药科学技术研究项目(2013-084) （2013-084）
