渔业现代化Issue(5)：44-48,5.DOI:10.3969/j.issn.1007-9580.2014.05.010
鳄鱼血蛋白酶解产物抗氧化特性和血管紧张素转化酶抑制活性研究
Study on the antioxidant properties and angiotensin-converting enzyme inhibitory activity of crocodile blood protein enzymatic hydrolysate
摘要
Abstract
The antioxidant properties and angiotensin-converting enzyme ( ACE) inhibitory activity of enzymatic hydrolysate from crocodile blood protein were analyzed. The crocodile plasma and blood cell protein were hydrolyzed by papain, and then antioxidant properties and ACE inhibition rate of enzymatic hydrolysate were measured by spectrophotometer and HPLC. Results showed that there were no statistical significance ( P>0.05) between the enzymatic hydrolysate of crocodile plasma and blood cell protein; the ABTS radical-scavenging ability of enzymatic hydrolysate from blood cell protein was higher than that from crocodile plasma protein from 0 to 5 mg/mL of protein concentration, and there were statistical significance at P<0.01 between the two enzymatic hydrolysate at 1 mg/mL;DPPH radical-scavenging ability of crocodile plasma protein enzymatic hydrolysate was enhanced with the increasing protein concentration during 0-5 mg/mL, but that of blood cell protein reached the highest ability at 4 mg/mL, then was declined;both the reducing power of two enzymatic hydrolysate increased evidently with the increasing protein concentration within 0-20 mg/mL, but there were no statistical significance (P>0.05) between them ; crocodile plasma and blood cell protein enzymatic hydrolysate both revealed good ability of ACE inhibition, the maximum inhibition of them can reach 75. 56 % and 86. 42 %, respectively. Therefore, the enzymatic hydrolysate of crocodile blood protein has ability of antioxidant and ACE inhibition.关键词
鳄鱼血/酶解产物/抗氧化特性/ACE抑制力Key words
crocodile blood/enzymatic hydrolysate/antioxidant properties/ACE inhibition分类
农业科技引用本文复制引用
黄和平,陈孙福,罗永康..鳄鱼血蛋白酶解产物抗氧化特性和血管紧张素转化酶抑制活性研究[J].渔业现代化,2014,(5):44-48,5.基金项目
科技部星火计划引导项目“鳄鱼肉松加工技术集成与示范” （）
