诊断学理论与实践Issue(1)：36-40,5.DOI:10.3969/j.issn.1671-2870.2015.01.009
MEK鄄ERK信号通路在全反式维A酸诱导急性早幼粒细胞白血病细胞分化中的作用研究
The role of MEK-ERK signaling pathway in all-trans retinoic acid induced differentiation of APL cells
摘要
Abstract
Objective: To study the role of MEK-ERK signaling pathway in ATRA-induced differentiation of APL cells and its potential mechanisms. Methods: APL cell line NB4 was used as in vitro model. The cell differentiation was evaluated by cell surface differentiation antigen CD11b, nitroblue-tetyrazolium (NBT) reduction assay and cellular mor-phology. The activation of MEK and ERK as well as the protein expression levels of PU.1, C/EBPβ, C/EBPε and PML-RARα were assayed by Western blotting. Results: MEK-ERK signaling pathway was activated at the early period of ATRA treatment and the activation persisted for 48 h. Attenuation of activation of MEK resulted in declination of percent-age of ATRA-induced CD11b positive cells from (87.50±4.16)%to (38.01±2.79)%. Meanwhile, A540 value of NBT reduc-tion assay was also decreased from 0.507±0.009 to 0.250±0.010. Both were statistically significant (P<0.01 and P<0.001 respectively). Furthermore, most of cells restored to characteristic morphology of APL cells. The increased protein levels of PU.1, C/EBPβ and C/EBPε induced by ATRA treatment were suppressed. Conclusions: ATRA could induce differentia-tion of APL cells through regulation of protein level of PU.1, C/EBPβand C/EBPεby MEK-ERK signaling pathway.关键词
急性早幼粒细胞性白血病/细胞分化/全反式维A酸/MEK-ERKKey words
Acute promyelocytic leukemia cells/Cell differentiation/All-trans retinoic acid/MEK-ERK分类
医药卫生引用本文复制引用
盖东征,翁香琴,盛燕,吴婧,蔡循..MEK鄄ERK信号通路在全反式维A酸诱导急性早幼粒细胞白血病细胞分化中的作用研究[J].诊断学理论与实践,2015,(1):36-40,5.基金项目
上海市自然科学基金项目 （）
