诊断学理论与实践Issue(2)：155-158,4.DOI:10.16150/j.1671-2870.2015.02.014
同型半胱氨酸对大鼠血管平滑肌细胞增殖及胶原分泌的影响
Effect of homocysteine on proliferation and production of collagens in rat vascular smooth muscle cells
摘要
Abstract
Objective: To investigate the effect of different concentrations of homocysteine (Hcy)on proliferation of rat vascular smooth cells (vSMCs) and correlations with production of collagen types Ⅰ, Ⅲ, Ⅳ and Ⅵ. Methods: Rat vascular smooth muscle cells (vSMCs) were treated with different concentrations of Hcy, and at different timepoints the cell viability was evaluated by MTT, cell proliferation by BrdU and flow cytometry, and collagens were measured by ELISA. Results: vSMCs proliferation increased significantly at concentrations of Hcy 0.025-0.500 mmol/L after 12, 48 h of treatment. Production of collagen types Ⅰ, Ⅳ were increased with the increase of concentrations of Hcy in a dose-de-pendent manner, while type Ⅲ only increased slightly without statistical significance. Type Ⅵ collagen was significantly reduced at high concentrations of Hcy in a dose-dependent manner. Conclusions:Proliferation of vSMCs could be induced by Hcy, and the present investigation suggests that Hcy might contribute to the accelerated progression of atherosclerotic lesions with collagen alterations, which led to plaque instability and vulnerability.关键词
同型半胱氨酸/动脉粥样硬化/胶原类型/斑块形成/平滑肌细胞Key words
Homocysteine/Atherosclerosis/Collagen type/Plaque formation/Smooth muscle cells分类
医药卫生引用本文复制引用
权薇薇,吴春芳,陆国平,徐志红..同型半胱氨酸对大鼠血管平滑肌细胞增殖及胶原分泌的影响[J].诊断学理论与实践,2015,(2):155-158,4.基金项目
上海市科委重大课题 （）
