中国病理生理杂志Issue(4)：584-591,8.DOI:10.3969/j.issn.1000-4718.2014.04.002
胡黄连苷Ⅱ对大鼠脑缺血损伤后髓鞘碱性蛋白表达的影响
Effect of picrosideⅡon expression of myelin basic protein after cerebral ischemia injury in rats
摘要
Abstract
AIM: To verify the neuroprotective effect and optimize the therapeutic dose and time window of picroside Ⅱon cerebral ischemic injury in rats .METHODS:The forebrain ischemia model was established by the method of bilateral common carotid artery occlusion ( BCCAO ) .The successful model rats were randomly divided into 16 groups according to orthogonal design and treated by intraperitoneal injection of picroside Ⅱat different ischemic time poinis and different doses .The changes of the nerve fiber myelin were observed by fast green staining .The immunohistochemical assay and Western blotting were used to quantitatively and qualitatively determine the expression of myelin basic protein (MBP). The mRNA level of MBP in the brain tissues was tested by reverse transcription polymerase chain reaction (RT-PCR).RE-SULTS:Picroside Ⅱ increased the expression of MBP and decreased demyelination after cerebral ischemic injury .The best therapeutic time window and dose were:(1) ischemia for 2.0 h with picrosideⅡat dose of 10 mg/kg according to the results of fast green staining;(2) ischemia for 2.0 h with the dose of 10 mg/kg according to the results of immunohisto-chemical assay;(3) ischemia for 2.0 h with the dose of 10 mg/kg according to the analysis of Western blotting;(4) is-chemia for 1.5 h with the dose of 20 mg/kg according to the detection of RT-PCR.CONCLUSION:Given the principle of the lowest therapeutic dose with the longest time window , the optimized therapeutic dose and time window for rat cerebral ischemic injury is intraperitoneal injection of picroside Ⅱat the doses of 10~20 mg/kg and the time window of ischemia for 1.5~2.0 h.关键词
胡黄连苷Ⅱ/脑缺血/大鼠/髓鞘碱性蛋白Key words
Picroside Ⅱ/Brain ischemia/Rats/Myelin basic protein分类
医药卫生引用本文复制引用
赵丽,郭云良..胡黄连苷Ⅱ对大鼠脑缺血损伤后髓鞘碱性蛋白表达的影响[J].中国病理生理杂志,2014,(4):584-591,8.基金项目
国家自然科学基金资助项目 （）
