中国病理生理杂志Issue(6)：968-974,7.DOI:10.3969/j.issn.1000-4718.2014.06.002
顺铂激活的低分化鼻咽癌细胞氯电流为非钙激活氯电流
Chloride currents activated by cisplatin in poorly differentiated naso-pharyngeal carcinoma cells are not Ca2+-activated chloride currents
摘要
Abstract
AIM:To investigate the type of chloride channel activated by cisplatin in poorly differentiated na -sopharyngeal carcinoma cells (CNE-2Z cells).METHODS:The technique of whole-cell patch-clamp was used to investi-gate the role of Ca 2+in the activation of cisplatin-activated chloride currents and to analyze the effect of hypertonic stress on these currents in CNE-2Z cells.RESULTS:Chloride currents were induced when the cells were exposed to the calcium -free cisplatin solution , showing the similar density to the currents induced by cisplatin with the presence of extracellular cal -cium.However , the latency and the peak time of cisplatin-activated currents in the absence of extracellular calcium were prolonged.The activation of cisplatin-activated chloride currents was insensitive to the depletion of intra-and extracellular calcium.Calcium channel antagonist nifedipine had no effect on the cisplatin -activated chloride currents , while hypertonic solution completely inhibited those currents .CONCLUSION:The cisplatin-activated chloride currents are independent on intra/extracellular calcium .The chloride channels activated by cisplatin are not calcium-activated chloride channels , but are probably volume-sensitive chloride channels .关键词
顺铂/鼻咽肿瘤/氯通道/钙Key words
Cisplatin/Nasopharyngeal neoplasms/Chloride channels/Calcium分类
医药卫生引用本文复制引用
阳小雅,陈丽新,刘梅,伍嘉宝,赖周易,汪源,范爱辉,朱林燕,毛建文,王立伟..顺铂激活的低分化鼻咽癌细胞氯电流为非钙激活氯电流[J].中国病理生理杂志,2014,(6):968-974,7.基金项目
国家自然科学基金资助项目（No．81173064；No．81272223；No．81273539）；教育部博士点基金资助项目（No．20124401110009）；广东省医学科学技术研究基金资助项目（ No．B2012187）；广州市科技计划项目（ No．2013J500015）；东莞市科技计划项目 （）
