中国病理生理杂志Issue(6)：975-981,7.DOI:10.3969/j.issn.1000-4718.2014.06.003
下调 p21活化蛋白激酶2表达对人乳腺癌细胞增殖和凋亡的影响
Effects of p21-activated protein kinase 2 down-regulation on proliferation and apoptosis of human breast cancer cells
摘要
Abstract
AIM:To study the effect of p21-activated protein kinase 2 (PAK2) knockdown by RNA interfer-ence on the proliferation and apoptosis of human breast cancer cells .METHODS:The short hairpin RNA ( shRNA) targe-ting PAK2 gene was designed and used for packing lentivirus in 293T cells.Human breast cancer MCF-7 cells were infected by the virus particles and PAK2 knockdown stable cell line was established by puromycin selection .The knockdown effi-ciency was assessed by Western blotting .The proliferation ability of MCF-7 cells was evaluated by CellTiter 96 AQueous and anchorage-independent growth assays .The cell apoptosis induced by staurosporine was detected by flow cytometry . RESULTS:The protein level of PAK2 was significantly suppressed after silencing of PAK2 gene in MCF-7 cells ( P<0.01).Furthermore, knockdown of PAK2 caused remarkable inhibition of the cell proliferation and colony formation (P<0.01).Staurosporine induced more apoptosis in the PAK2 knockdown cells compared with the control cells (P<0.01). CONCLUSION:Knockdown of PAK2 inhibits the proliferation of MCF-7 cells and increases the sensitivity of chemothera-peutic drug-induced cell apoptosis , suggesting that PAK2 might be a new therapeutic target in breast cancer treatment .关键词
p21活化蛋白激酶2/乳腺肿瘤/RNA干扰/细胞增殖/细胞凋亡Key words
p21-activated protein kinase 2/Breast neoplasms/RNA interference/Cell proliferation/Apoptosis分类
医药卫生引用本文复制引用
李翔,张晓艳,赵继敏,刘康栋,赵明耀,董子明..下调 p21活化蛋白激酶2表达对人乳腺癌细胞增殖和凋亡的影响[J].中国病理生理杂志,2014,(6):975-981,7.基金项目
国家自然科学基金资助项目（No．31301144）；国家教育部博士学科点专项科研基金（No．20134101120011）；河南省教育厅科学技术研究重点项目 （）
