中国病理生理杂志Issue(6)：1066-1069,4.DOI:10.3969/j.issn.1000-4718.2014.06.018
米诺环素调控P2X7受体抑制BV-2细胞活化的研究
Minocycline inhibits BV-2 cell activation by regulating P2X7 receptor
摘要
Abstract
AIM: To explore the role of P2X7 receptor in inhibition of lipopolysaccharide (LPS)-stimulated BV-2 cell activation by minocycline .METHODS:BV-2 cells were divided into 5 groups:control group, LPS group, LPS+0.1 μmol/L Mino group, LPS+1 μmol/L Mino group and LPS+10 μmol/L Mino group.The expression of P2X7 re-ceptor was determined by real-time PCR and Western blotting .The levels of TNF-αand IL-1βin the microglia culture su-pernatants were measured by ELISA .The morphological changes of the cells were also observed .RESULTS: After ex-posed to LPS, the expression of P2X7 receptor increased in BV-2 cells at mRNA and protein levels .The concentrations of TNF-αand IL-1βin the microglia culture supernatants also increased .Meanwhile, 0.1~10μmol/L minocycline inhibited those changes in a dose-dependent manner .CONCLUSION:Minocycline inhibits the activation of microglia .The mecha-nism may be related to the P2X7 receptor.关键词
米诺环素/受体, P2 X7/肿瘤坏死因子α/白细胞介素1βKey words
Minocycline/Receptors, P2X7/Tumor necrosis factor α/Interleukin 1β分类
医药卫生引用本文复制引用
刘淑琼,杨炼红,蒋龙元,叶晋豪..米诺环素调控P2X7受体抑制BV-2细胞活化的研究[J].中国病理生理杂志,2014,(6):1066-1069,4.基金项目
中山大学学生科研基金资助项目（No．55）；广东省大学生创新实验项目 （）
