中国病理生理杂志Issue(7)：1184-1191,8.DOI:10.3969/j.issn.1000-4718.2014.07.006
人结肠癌细胞系 SP 细胞 microRNA 表达谱的初步分析
Priliminary analysis of microRNA expression profiles of side population cells in human colon cancer cell lines
摘要
Abstract
AIM:To investigate the role of side population ( SP) cells in multidrug resistance of colon cancer cells and microRNA biomarkers of SP cells in colon cancer cells .METHODS:SP cells in colon cancer cells were sorted by flow cytometry .The cell viability was measured by MTT method .MicroRNA expression profiles were detected by mi-croRNA chip.MicroRNA expression was verified by real-time PCR.RESULTS:The ratios of SP cells in HCT-15, HT-29 and LoVo colon cancer cell lines were 16.75%, 13.02%and 9.52%, respectively.In all 3 colon cancer cell lines, IC50 of the antitumor drugs including 5-fluorouracil , oxaliplatin and adriamycin for the SP cells were significantly higher than those for non-SP cells (P<0.05).MicroRNA profiling showed that miR-5000-3p, miR-5009-3p and miR-552 were all up-regulated in the SP cells of all 3 colon cancer cell lines .This result was verified by real-time PCR.CONCLUSION:miR-5000-3p, miR-5009-3p and miR-552 are all up-regulated in the SP cells of colon cancer cell lines , and may be the poten-tial microRNA biomarkers of SP cells in colon cancer .关键词
结肠肿瘤/侧群细胞/微小RNA/miR-5000-3p/miR-5009-3p/miR-552Key words
Colonic neoplasms/Side population cells/MicroRNA/miR-5000-3p/miR-5009-3p/miR-552分类
医药卫生引用本文复制引用
夏忠胜,钟娃,于涛,练国达,周慧敏,陈广成..人结肠癌细胞系 SP 细胞 microRNA 表达谱的初步分析[J].中国病理生理杂志,2014,(7):1184-1191,8.基金项目
广东省自然科学基金资助项目 （）
