中国病理生理杂志Issue(8)：1493-1496,4.DOI:10.3969/j.issn.1000-4718.2014.08.027
慢病毒介导的 CCN1基因对大鼠骨髓间充质干细胞生长和迁移的影响
Lentivirus mediated CCN1 gene on growth and migration of rat bone marrow mesenchymal stem cells
摘要
Abstract
[ABSTRACT]AIM:Toinvestigatetheroleofcysteine-rich61(Cyr61/CNN1)inproliferationandmigrationof bone marrow mesenchymal stem cells ( BMSCs ) .METHODS: The lentiviral vector carrying CCN 1 ( Lenti-GFP-CCN1 ) was constructed and then transfected into the rat BMSCs .The cells were divided into non-transfection group , transfection group ( transfected with Lenti-GFP-CCN1 ) and negative control group ( Lenti-GFP ) .The fluorescence intensity of the transfected BMSCs was observed under inverted fluorescence microscope .The effects of CCN1 on the proliferation and mi-gration of BMSCs were detected by MTT assay and scratch wound healing assay .RESULTS:The proliferation of BMSCs transfected with Lenti-GFP CCN1 had no significant difference compared with negative control group and control group .The width/thickness ratio of migrated BMSCs in wound healing was significantly higher in Lenti-GFP-CCN1 group than that in negative control group and control group (P<0.05).CONCLUSION:Exogenous CCN1 promotes the migration of BMSCs.关键词
CCN1蛋白质/骨髓间充质干细胞/慢病毒Key words
CCN1 protein/Bone marrow mesenchymal stem cells/Lentivirus分类
医药卫生引用本文复制引用
孙湛,巩雪俐,冉新建,马琪,龙梅..慢病毒介导的 CCN1基因对大鼠骨髓间充质干细胞生长和迁移的影响[J].中国病理生理杂志,2014,(8):1493-1496,4.基金项目
新疆维吾尔自治区自然科学基金资助项目（ No．2012211B14）；中国博士后科学基金面上项目 （）
