中国病理生理杂志Issue(9)：1622-1632,11.DOI:10.3969/j.issn.1000-4718.2014.09.014
辅酶Q10与美满霉素联合干预1-甲基-4-苯基吡啶诱导的偏侧帕金森病大鼠模型研究
Integrative therapy using coenzyme Q10 and minocycline in 1-methyl-4-phenylpyridinium-induced hemiparkinson rats
高俊鹏 1周子懿 2向军 3陈依萍 3蔡定芳3
作者信息
- 1. 广州医科大学附属广州市第一人民医院中西医结合科，广东广州510160
- 2. 广州中医药大学附属第二临床医学院脑病一科，广东广州510120
- 3. 复旦大学附属中山医院中西医结合科，上海200032
- 折叠
摘要
Abstract
AIM:To investigate the integrative treatment of both coenzyme Q 10 ( CoQ10 ) and minocycline in the rats intranigrally intoxicated with 1-methyl-4-phenylpyridinium ( MPP+) .METHODS:The rat model of Parkinson disease ( PD) was established by intranigral microinjection of MPP +.The degree of microglial activation was measured by immuno-fluorescent density of OX-42 ( a microglia marker ) in the substantia nigra ( SN) .The number of viable dopaminergic neurons was determined by counting the tyrosine hydroxylase ( TH) positive neurons in the SN .The behavioral performances were re-vealed with the number of apomorphine-induced rotations , score of forelimb akinesia and vibrissae-elicited forelimb placing a-symmetry.RESULTS:Pretreatment with CoQ10 or intracerebroventricular (icv) posttreatment with minocycline alone pro-vided partial attenuation against MPP +-induced locomotor defects .Integrative therapy provided enhanced beneficial effects , and resulted in a significant attenuation of locomotor disability than any single therapy (all P<0.01).The results of immu-nohistological analysis showed that the TH positive neurons were maximally protected by integrative therapy compared with minocycline group and CoQ 10 group (P<0.01) .CONCLUSION:The integrative therapy of CoQ 10 combined with minocy-cline may offer additional therapeutic benefit to MPP +-induced hemiparkinson rat model .Such neuroprotective strategy of tar-geting different aspect of the neurodegenerative phenotypes may highlight a new therapeutic strategy for future management of PD.关键词
帕金森病/线粒体复合体I/辅酶Q10/小神经胶质细胞/美满霉素/多巴胺能神经元Key words
Parkinson disease/Mitochondrial complex I/Coenzyme Q10/Microglia/Minocycline/Dopami-nergic neuron分类
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高俊鹏,周子懿,向军,陈依萍,蔡定芳..辅酶Q10与美满霉素联合干预1-甲基-4-苯基吡啶诱导的偏侧帕金森病大鼠模型研究[J].中国病理生理杂志,2014,(9):1622-1632,11.
