中国病理生理杂志Issue(10)：1753-1759,7.DOI:10.3969/j.issn.1000-4718.2014.10.005
五步蛇毒 PCA 改善脓毒症大鼠的血管低反应性
Improvement of vascular hyporesponsiveness in rats with sepsis by pro-tein C activator from Agkistrodon acutus venom
摘要
Abstract
AIM: To investigate the effects of protein C activator (PCA) from Agkistrondon acutus venom ( AAV) on the tension of thoracic aorta rings isolated from the rats with sepsis.METHODS:The model of sepsis was es-tablished by intraperitoneal injection of lipopolysaccharide ( LPS) .SD rats were randomly divided to 6 groups ( n=6 ):sham group, LPS group, PCA intervention group (LPS+PCA, PCA at doses of 0.1 mg/kg, 0.3 mg/kg and 0.6 mg/kg) and LPS+polymyxin B (at dose of 0.2 mg/kg) group.Using perfusion experiment in vitro, the tension of the aortic rings was measured by biological signal analytical system.RESULTS:The values of MABP, HR, LVDP and ±dp/dtmax were significantly lower in LPS group than those in sham group and LPS+PCA groups.Compared with sham group, the relaxa-tion response to acetylcholine ( ACh) and the contractile response of aorta rings induced by phenylephrine ( Phe) were sig-nificantly decreased in LPS group, which were increased significantly in PCA intervention group ( especially at dose of 0.6 mg/kg) compared with LPS group.The dose-response curve of aorta contraction with denuded endothelium induced by Phe shifted down significantly in LPS group compared with sham group, and no significant difference between LPS group and PCA intervention group was observed.Also no statistical difference was found in non-endothelium dependent relaxation of aortic rings induced by sodium nitroprusside among the groups.Pretreatment of N-nitro-L-arginine methl ester and methyl-ene blue increased the contraction amplitude of aortic rings induced by Phe.CONCLUSION:PCA from AAV effectively reverses the hypoergia of the vessels in rats with sepsis through protecting vascular endothelium, the mechanism of which may be mediated by inhibiting NO-GC-cGMP signal transduction pathway.关键词
五步蛇/脓毒症/蛋白C激活剂/主动脉Key words
Agkistrondon acutus/Sepsis/Protein C activator/Aorta分类
医药卫生引用本文复制引用
包鹏举,孙瑶,王海华,张根葆,胡钱国,蒋佳珅..五步蛇毒 PCA 改善脓毒症大鼠的血管低反应性[J].中国病理生理杂志,2014,(10):1753-1759,7.基金项目
安徽省教育厅高等学校自然科学基金一般项目（ No．KJ2013B317）；皖南医学院中青年科研基金自然科学类项目（No．WK201208）；安徽省教育厅高等学校自然科学基金重点项目（No．KJ2013A251）；国家大学生创新创业训练计划项目 （）
